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Co-fermentation of waste activated sludge with food waste for short-chain fatty acids production: effect

Leiyu FENG, Yuanyuan YAN, Yinguang CHEN

《环境科学与工程前沿（英文）》 2011年 第5卷 第4期   页码 623-632 doi: 10.1007/s11783-011-0334-2

摘要： Effect of pH ranging from 4.0 to 11.0 on co-fermentation of waste activated sludge (WAS) with food waste for short-chain fatty acids (SCFAs) production at ambient temperature was investigated in this study. Experimental results showed that the addition of food waste significantly improved the performance of WAS fermentation system, which resulted in the increases of SCFAs production and substrate reduction. The SCFAs production at pH 6.0, 7.0, 8.0, and 9.0 and fermentation time of 4 d was respectively 5022.7, 6540.5, 8236.6, and 7911.7 mg COD·L , whereas in the blank tests (no pH adjustment, pH 8.0 (blank test 1), no food waste addition, pH 8.0 (blank test 2), and no WAS addition (blank test 3)) it was only 1006.9, 971.1, and 1468.5 mg COD·L , respectively. The composition of SCFAs at pH from 6.0 to 9.0 was also different from other conditions and propionic acid was the most prevalent SCFA, which was followed by acetic and n-butyric acids, while acetic acid was the top product under other conditions. At pH 8.0 a higher volatile suspended solids (VSS) reduction of 16.6% for the mixture of WAS and food waste than the sole WAS indicated a synergistic effect existing in fermentation system with WAS and food waste. The influence of pH on the variations of nutrient content was also studied during anaerobic fermentation of the mixture of WAS and food waste at different pH conditions. The release of increased with fermentation time at all pH values investigated except 4.0, 5.0 and in blank test one. The concentrations of soluble phosphorus at acidic pHs and in the blank test one were higher than those obtained at alkaline pHs. Ammonia and phosphorus need to be removed before the SCFAs-enriched fermentation liquid from WAS and food waste was used as the carbon source.

关键词： waste activated sludge (WAS)     food waste     co-fermentation     short-chain fatty acids (SCFAs)     pH     synergistic effect    

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Enhanced hydrogen production in microbial electrolysis through strategies of carbon recovery from alkaline/thermal treated sludge

Ling Wang, Chunxue Yang, Sangeetha Thangavel, Zechong Guo, Chuan Chen, Aijie Wang, Wenzong Liu

《环境科学与工程前沿（英文）》 2021年 第15卷 第4期 doi: 10.1007/s11783-020-1348-4

摘要： Abstract • High hydrogen yield is recovered from thermal-alkaline pretreated sludge. • Separating SFL by centrifugation is better than filtration for hydrogen recovery. • The cascaded bioconversion of complex substrates in MECs are studied. • Energy and electron efficiency related to substrate conversion are evaluated. The aim of this study was to investigate the biohydrogen production from thermal (T), alkaline (A) or thermal-alkaline (TA) pretreated sludge fermentation liquid (SFL) in a microbial electrolysis cells (MECs) without buffer addition. Highest hydrogen yield of 36.87±4.36 mgH2/gVSS (0.026 m3/kg COD) was achieved in TA pretreated SFL separated by centrifugation, which was 5.12, 2.35 and 43.25 times higher than that of individual alkaline, thermal pretreatment and raw sludge, respectively. Separating SFL from sludge by centrifugation eliminated the negative effects of particulate matters, was more conducive for hydrogen production than filtration. The accumulated short chain fatty acid (SCFAs) after pretreatments were the main substrates for MEC hydrogen production. The maximum utilization ratio of acetic acid, propionic acid and n-butyric acid was 93.69%, 90.72% and 91.85%, respectively. These results revealed that pretreated WAS was highly efficient to stimulate the accumulation of SCFAs. And the characteristics and cascade bioconversion of complex substrates were the main factor that determined the energy efficiency and hydrogen conversion rate of MECs.

关键词： Waste activated sludge (WAS)     Short chain fatty acids (SCFAs)     Hydrogen     Pretreatment     Microbial electrolysis cells (MECs)    

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Optimization of pretreatment of Jatropha oil with high free fatty acids for biodiesel production

Supriyono SUWITO, Giuliano DRAGONE, Hary SULISTYO, Bardi MURACHMAN, Suryo PURWONO, José TEIXEIRA

《化学科学与工程前沿（英文）》 2012年 第6卷 第2期   页码 210-215 doi: 10.1007/s11705-012-1282-5

摘要： A central composite rotatable design and response surface methodology were used in order to investigate the individual and combined effects of the ethanol-to-oil ratio, H SO concentration, temperature and time of reaction on the reduction of free fatty acid (FFA) in jatropha oil. A quadratic polynomial model relating the reaction variables with FFA reduction was developed, presenting a good coefficient of determination ( = 0.893). For reducing FFA to less than 1%, the optimal combination was found to be 0.62 v·v ethanol-to-oil ratio (14.9 v·v ethanol-to-FFA ratio), 1.7% v·v H SO concentration, and 79 min reaction time at a reaction temperature of 54°C. These results are of great relevance to maximize methyl esters formation by transesterification using an alkaline catalyst.

关键词： biodiesel     biofuel     esterification     free fatty acids     jatropha curcas oil    

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Inhibition character of crotonaldehyde manufacture wastewater on biological acidification

《环境科学与工程前沿（英文）》 2021年 第15卷 第6期 doi: 10.1007/s11783-021-1403-9

摘要：

• The inhibition of the main organic pollutions in CMW was demonstrated.

关键词： Crotonaldehyde manufacture wastewater     Biological acidification     Volatile fatty acids     Inhibition     Toxic units    

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Mechanistic and therapeutic advances in non-alcoholic fatty liver disease by targeting the gut microbiota

Ruiting Han, Junli Ma, Houkai Li

《医学前沿（英文）》 2018年 第12卷 第6期   页码 645-657 doi: 10.1007/s11684-018-0645-9

摘要： Non-alcoholic fatty liver disease (NAFLD) is one of the most common metabolic diseases currently in the context of obesity worldwide, which contains a spectrum of chronic liver diseases, including hepatic steatosis, non-alcoholic steatohepatitis and hepatic carcinoma. In addition to the classical “Two-hit” theory, NAFLD has been recognized as a typical gut microbiota-related disease because of the intricate role of gut microbiota in maintaining human health and disease formation. Moreover, gut microbiota is even regarded as a “metabolic organ” that play complementary roles to that of liver in many aspects. The mechanisms underlying gut microbiota-mediated development of NAFLD include modulation of host energy metabolism, insulin sensitivity, and bile acid and choline metabolism. As a result, gut microbiota have been emerging as a novel therapeutic target for NAFLD by manipulating it in various ways, including probiotics, prebiotics, synbiotics, antibiotics, fecal microbiota transplantation, and herbal components. In this review, we summarized the most recent advances in gut microbiota-mediated mechanisms, as well as gut microbiota-targeted therapies on NAFLD.

关键词： gut microbiota     NAFLD     obesity     insulin resistance     bile acids     probiotic    

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微生物电合成中链脂肪酸 Article

褚娜, 郝雯, 吴清莲, 梁勤军, 蒋永, 梁鹏, 任智勇, 曾建雄

《工程（英文）》 2022年 第16卷 第9期   页码 141-153 doi: 10.1016/j.eng.2021.03.025

摘要：

微生物电合成（MES）使用微生物催化剂和电化学手段促进CO2生物转化，也应用于有机废物生物炼制。本文总结了MES 利用CO2 和有机废物产中链脂肪酸（MCFA）的研究现状与发展趋势，对传统发酵产MCFA的基本原理和研究进展进行了归纳。首先，概述了MES产MCFA的相关报道，重点介绍了多电子供体（ED）策略。其次，讨论了MES利用CO2产MCFA面临的挑战，并针对产甲烷抑制、产乙酸菌三磷酸腺苷（ATP）限制、产有机溶剂阶段提供ED有限进行了详细阐述。再次，分析了电化学手段促进有机废物生物炼制产MCFA的潜力。最后，从多级反应、底物供应、产物提取、微生物代谢路径等角度展望了未来发展方向。

关键词： 电发酵     微生物电化学     产乙酸菌     碳链延长     电活性微生物    

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Effects of fatty acid chain length and degree of unsaturation on the surface activities of monoacyl trehaloses

Yue-E SUN, Wenshui XIA, Xueyan TANG, Zhiyong HE, Jie CHEN,

《化学科学与工程前沿（英文）》 2009年 第3卷 第4期   页码 407-412 doi: 10.1007/s11705-009-0255-9

摘要： The surface properties of monoacyl trehaloses with different acyl chains were investigated at 30°C, 40°C, 50°C, and 60°C. Monoacyl trehaloses were enzymatically synthesized and purified with silica gel column chromatography and semi-preparative high-performance liquid chromatography (HPLC), and the purity of products was identified by mass spectrometry (MS) and nuclear magnetic resonance (NMR). The surface tension of monoacyl trehalose in pure water was measured using Doüy ring method at different temperatures. The critical micelle concentrations (CMC), surface tension at the CMC, , and residual area per molecule, , were estimated from the curves. The CMC value of unsaturated monoacyl trehalose was affected by both the degree of unsaturation and the acyl chain length, and the effect of chain length on the CMC value was much stronger than that of the unsaturation degree. However, there was no significant dependency of the value and a values on the chain length or the unsaturated degree.

关键词： high-performance liquid     tension     different     spectrometry     chromatography    

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Expression and characterization of a CALB-type lipase from

Jiang-Wei Shen, Xue Cai, Bao-Juan Dou, Feng-Yu Qi, Xiao-Jian Zhang, Zhi-Qiang Liu, Yu-Guo Zheng

《化学科学与工程前沿（英文）》 2020年 第14卷 第5期   页码 868-879 doi: 10.1007/s11705-019-1889-x

摘要： A lipase from SRZ2 (SRL) with 73% amino acid sequence identity to lipase B (CALB) was cloned and overexpressed in . The recombinant SRL showed a preference for short-chain -nitrophenyl esters. It achieved maximum activity at pH 8.0 and 65°C for -nitrophenyl hexanoate (C6) with and / values of 0.14 mmol∙L and 1712 min ∙mmol∙L at 30°C, respectively. SRL displayed excellent thermostability and pH stability, retaining more than 79% of its initial activity after incubation at 60°C for 72 h and 75% at pH 3 to 11 for 72 h. It also maintained most of its activity in the presence of inhibitors and detergents except sodium dodecyl sulfate, and it tolerated organic solvents. SRL was covalently immobilized and successfully used for ethyl hexanoate synthesis in cyclohexane or in a solvent-free system with a high conversion yield (>95%). Furthermore, high conversion yield was also achieved for the synthesis of various short-chain flavor esters when high substrate concentrations of 2 mol∙L were applied. This study indicated that a CALB-type lipase from SRZ2 showed great potential in organic ester synthesis.

关键词： lipase     Sporisorium reilianum     biochemical characterization     short-chain flavor ester     solvent-free system    

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Insulin resistance and the metabolism of branched-chain amino acids

null

《医学前沿（英文）》 2013年 第7卷 第1期   页码 53-59 doi: 10.1007/s11684-013-0255-5

摘要：

Insulin resistance (IR) is a key pathological feature of metabolic syndrome and subsequently causes serious health problems with an increased risk of several common metabolic disorders. IR related metabolic disturbance is not restricted to carbohydrates but impacts global metabolic network. Branched-chain amino acids (BCAAs), namely valine, leucine and isoleucine, are among the nine essential amino acids, accounting for 35% of the essential amino acids in muscle proteins and 40% of the preformed amino acids required by mammals. The BCAAs are particularly responsive to the inhibitory insulin action on amino acid release by skeletal muscle and their metabolism is profoundly altered in insulin resistant conditions and/or insulin deficiency. Although increased circulating BCAA concentration in insulin resistant conditions has been noted for many years and BCAAs have been reported to be involved in the regulation of glucose homeostasis and body weight, it is only recently that BCAAs are found to be closely associated with IR. This review will focus on the recent findings on BCAAs from both epidemic and mechanistic studies.

关键词： branched-chain amino acids     leucine     isoleucine     valine     insulin resistance    

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Identification of Legionella species by the composition of cellular fatty acids

Cun-Lei FANG MD, Zhao-Hui HU PhD, Qing-Yi ZHU MD, Ya-Jun SONG PhD, Ya-Fang TAN PhD, Rui-Fu YANG PhD,

《医学前沿（英文）》 2010年 第4卷 第2期   页码 208-215 doi: 10.1007/s11684-010-0031-8

摘要： One hundred and fifty-three isolates from the environment and 36 reference strains of the were studied with regards to their composition of cellular fatty acids as determined by gas chromatography, and then were classified into 41 groups by numerical analysis. Most reference strains formed only a single group, except , , , , and , which were clustered into two or three groups. Even serological types of could be clearly identified. Therefore, in this study, numerical analysis of cellular fatty acid composition is an effective method for identifying species.

关键词： Legionella     numerical analysis     identification     fatty acids composition    

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Analysis of Brucella cellular fatty acids

Zhen-Xiang ZHAO MS, Hai JIANG MD, Bu-Yun CUI MS, Hong-Yan ZHAO, Dong-Ri PIAO MS, Lan-Yu LI, Su-Zhen HAO MS,

《医学前沿（英文）》 2010年 第4卷 第2期   页码 216-219 doi: 10.1007/s11684-010-0037-2

摘要： To acquire data of Brucella cellular fatty acids (CFAs) and probe into the possibility of utilizing CFAs information in typing Brucella, 19 reference strains were subjected to CFAs study. After all strains were inoculated on Brucella Agar plates, the cells were harvested, saponificated, methylated and extracted to provide fatty acid methylesters for gas chromatography (GC) analysis. Based on the CFAs data matrix, a dendrogram of 19 reference strains was generated by SPSS11.5 software package. The results showed that 19 reference strains were divided into five clusters: cluster 1 included (bv. 1, 2, 3, 5) and ; cluster 2 included (bv. 3, 4, 5, 6) and (bv. 1, 2, 3); cluster 3 included (bv. 1, 2, 7, 9) and ; cluster 4 was (bv. 4); and cluster 5 was . Typing Brucella by GC analysis of CFAs is a good method to reflect drug resistance of Brucella, and the classification is beneficial for clinical therapy. It also provides a new result of typing and demonstrates that the traditional classification is not completely reasonable. CFAs analysis may identify (bv. 4) and .

关键词： Brucella     gas chromatography     cellular fatty acids     dendrogram     typing    

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Machine learning enabled prediction and process optimization of VFA production from riboflavin-mediated sludge fermentation

《环境科学与工程前沿（英文）》 2023年 第17卷 第11期 doi: 10.1007/s11783-023-1735-8

摘要：

● Data-driven approach was used to simulate VFA production from WAS fermentation.

关键词： Machine learning     Volatile fatty acids     Riboflavin     Waste activated sludge     eXtreme Gradient Boosting    

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Influence of short chain ceramides and lipophilic penetration enhancers on the nano-structure of stratum

Annett SCHROETER, Tanja ENGELBRECHT, Reinhard H. H. NEUBERT

《化学科学与工程前沿（英文）》 2013年 第7卷 第1期   页码 29-36 doi: 10.1007/s11705-013-1302-0

摘要： Oriented stratum corneum model lipid membranes were used to study the influence of the short chain ceramides (CER)[NP] and [AP] as well as the impact of the lipophilic penetration enhancer molecules oleic acid (OA) and isopropyl myristate (IPM) on the lipid nanostructure. The influence of the enhancer molecules were studied using specifically deuterated OA and IPM and neutron diffraction. H NMR spectroscopy was used to study the impact of the ceramides’ degree of order within the stratum corneum model lipid membranes. It was found that CER[NP] forms two very stable phases with high resistance against temperature increase. Phase B showed unusual hydration behavior as no water uptake of this phase was observed. The H NMR spectroscopic measurements showed that CER[NP] based ternary model system had a higher state of lamellar order in comparison to CER[AP] based lipid matrix. The studies confirmed that the short chain ceramides, particularly CER[NP], have a very high impact on the integrity of the Stratum corneum lipid bilayers. The penetration enhancer OA has not influenced the repeat distance of the model membrane based on CER[AP], and was not able to induce a phase separation in the investigated lipid matrix. However, a disorder and a fluidisation of the model membranes were observed when OA was incorporated. IPM showed the same effect but two phases (assigned as phase A and B) appeared, when IPM was used as penetration enhancer and incorporated into the model membrane. Furthermore, two arrangements of IPM were identified in phase A using deuterated IPM. A model of the nanostructure of the Stratum corneum lipid membranes is presented.

关键词： nano-structure of the stratum corneum     ceramide     penetration enhancer     model membrane     neutron diffraction     2H NMR spectroscopy    

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New insights into different surfactants’ impacts on sludge fermentation: Focusing on the particular metabolic processes and microbial genetic traits

《环境科学与工程前沿（英文）》 2022年 第16卷 第8期 doi: 10.1007/s11783-022-1527-6

摘要：

• The promoting effects for VFA generation follow the order of APG>SDBS>HTAB.

关键词： Waste activated sludge (WAS)     Volatile fatty acids (VFA)     Surfactant types     Functional microorganisms     Metabolic activity upregulation    

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Influencing mechanism of high solid concentration on anaerobic mono-digestion of sewage sludge without agitation

Yuyao ZHANG,Huan LI,Can LIU,Yingchao CHENG

《环境科学与工程前沿（英文）》 2015年 第9卷 第6期   页码 1108-1116 doi: 10.1007/s11783-015-0806-x

摘要： High-solids anaerobic digestion of sewage sludge was a promising process, but high solid concentration negatively influenced methane production. The influencing mechanism was systematically analyzed in this study through a series of static anaerobic digestion experiments at total solids (TS) contents of 3%–15%. The results showed that TS 6% was the boundary between low-solids and high-solids anaerobic digestion, and the accumulative methane yield decreased exponentially when TS increased from 6% to 15%. The performance of anaerobic digestion was directly determined by the efficiency of mass transfer, and the relation between methane yield and sludge diffusive coefficients was well described by a power function. Thus, the increasing TS resulted in an exponential increase in sludge viscosity but an exponential decrease in diffusive coefficient. The blocked mass transfer led to the accumulation of volatile fatty acids (VFAs) and free ammonia. Acetic metabolism was the main process, whereas butyric and propionic metabolisms occurred at the initial stage of high-solids anaerobic digestion. The concentration of VFAs reached the maximum at the initial stage, which were still lower than the threshold influencing methanogens. The concentration of free ammonia increased gradually, and the methanogenesis was inhibited when free ammonia nitrogen exceeded 50 mg·L . Consequently, the deterioration of high-solids anaerobic digestion was related to the blocked mass transfer and the resulting ammonia accumulation.

关键词： anaerobic digestion     methane     sewage sludge     volatile fatty acids     free ammonia    

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标题 作者 时间 类型 操作

Co-fermentation of waste activated sludge with food waste for short-chain fatty acids production: effect

Leiyu FENG, Yuanyuan YAN, Yinguang CHEN

期刊论文

Enhanced hydrogen production in microbial electrolysis through strategies of carbon recovery from alkaline/thermal treated sludge

Ling Wang, Chunxue Yang, Sangeetha Thangavel, Zechong Guo, Chuan Chen, Aijie Wang, Wenzong Liu

期刊论文

Optimization of pretreatment of Jatropha oil with high free fatty acids for biodiesel production

Supriyono SUWITO, Giuliano DRAGONE, Hary SULISTYO, Bardi MURACHMAN, Suryo PURWONO, José TEIXEIRA

期刊论文

Inhibition character of crotonaldehyde manufacture wastewater on biological acidification

期刊论文

Mechanistic and therapeutic advances in non-alcoholic fatty liver disease by targeting the gut microbiota

Ruiting Han, Junli Ma, Houkai Li

期刊论文

微生物电合成中链脂肪酸

褚娜, 郝雯, 吴清莲, 梁勤军, 蒋永, 梁鹏, 任智勇, 曾建雄

期刊论文

Effects of fatty acid chain length and degree of unsaturation on the surface activities of monoacyl trehaloses

Yue-E SUN, Wenshui XIA, Xueyan TANG, Zhiyong HE, Jie CHEN,

期刊论文

Expression and characterization of a CALB-type lipase from

Jiang-Wei Shen, Xue Cai, Bao-Juan Dou, Feng-Yu Qi, Xiao-Jian Zhang, Zhi-Qiang Liu, Yu-Guo Zheng

期刊论文

Insulin resistance and the metabolism of branched-chain amino acids

null

期刊论文

Identification of Legionella species by the composition of cellular fatty acids

Cun-Lei FANG MD, Zhao-Hui HU PhD, Qing-Yi ZHU MD, Ya-Jun SONG PhD, Ya-Fang TAN PhD, Rui-Fu YANG PhD,

期刊论文

Analysis of Brucella cellular fatty acids

Zhen-Xiang ZHAO MS, Hai JIANG MD, Bu-Yun CUI MS, Hong-Yan ZHAO, Dong-Ri PIAO MS, Lan-Yu LI, Su-Zhen HAO MS,

期刊论文

Machine learning enabled prediction and process optimization of VFA production from riboflavin-mediated sludge fermentation

期刊论文

Influence of short chain ceramides and lipophilic penetration enhancers on the nano-structure of stratum

Annett SCHROETER, Tanja ENGELBRECHT, Reinhard H. H. NEUBERT

期刊论文

New insights into different surfactants’ impacts on sludge fermentation: Focusing on the particular metabolic processes and microbial genetic traits

期刊论文

Influencing mechanism of high solid concentration on anaerobic mono-digestion of sewage sludge without agitation

Yuyao ZHANG,Huan LI,Can LIU,Yingchao CHENG

期刊论文